2016 119:37–44.įox CB, Sivananthan SJ, Mikasa TJ, Lin S, Parker SC. A versatile, quantitative analytical method for pharmaceutical relevant lipids in drug delivery systems. Jeschek D, Lhota G, Wallner J, Vorauer-Uhl K. HPLC analysis as a tool for assessing targeted liposome composition. Oswald M, Platscher M, Geissler S, Goepferich A. In vivo toxicity of cationic micelles and liposomes. Knudsen KB, Northeved H, Kumar PE, Permin A, Gjetting T, Andresen TL, et al. Effect of the surface charge of liposomes on their uptake by angiogenic tumor vessels. Krasnici S, Werner A, Eichhorn ME, Schmitt-Sody M, Pahernik SA, Sauer B, et al. Toxicological considerations when creating nanoparticle-based drugs and drug delivery systems. Sharma A, Madhunapantula SV, Robertson GP. Liposomal drug delivery systems: from concept to clinical applications. Pharmaceutical liposomal drug delivery: a review of new delivery systems and a look at the regulatory landscape. New developments in liposomal drug delivery. Advances and challenges of liposome assisted drug delivery. Sercombe L, Veerati T, Moheimani F, Wu SY, Sood AK, Hua S. Hydration of polyethylene glycol-grafted liposomes. Tirosh O, Barenholz Y, Katzhendler J, Priev A. Interaction of peg-phospholipid conjugates with phospholipid-implications in liposomal drug-delivery. Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential. Effect of zeta potential on the properties of nano-drug delivery systems-a review (part 2). Effect of zeta potential on the properties of nano-drug delivery systems-a review (part 1). Effect of nanoparticle concentration on zeta-potential measurement results and reproducibility. Progress in nanoparticles characterization: sizing and zeta potential measurement. Malvern Zeta potential-an introduction in 30 minutes [Available from. Zeta potential in colloid science: principles and applications. Malvern zeta nano series [Available from. Singapore: World Scientific Publishing Ltd. 1: Key Considerations for Nanoparticle Characterization Prior to Immunotoxicity Studies. Handbook of Immunological properties of engineered nanomaterials. In: Dobrovolskaia MA, McNeil SE, editors. Importance of physicochemical characterization prior to immunological studies. Targeted polymeric therapeutic nanoparticles: design, development and clinical translation. Kamaly N, Xiao ZY, Valencia PM, Radovic-Moreno AF, Farokhzad OC. Factors affecting the clearance and biodistribution of polymeric nanoparticles. 2012 14:1–16.Īlexis F, Pridgen E, Molnar LK, Farokhzad OC. The effect of nanoparticle size, shape, and surface chemistry on biological systems. Very small amounts of the lipid-PEG (<0.2 mol%) were found to impart stability to the DOTAP- and DOPS-containing liposomes without significantly affecting other physicochemical properties of the formulation, providing a simple approach to making stable liposomes with cationic and anionic surface charge.Īlbanese A, Tang PS, Chan WCW. In addition, cationic and anionic liposomes were titrated with up to two mole percent of the neutral lipid 1,2-distearoyl- sn-glycero-3-phosphoethanolamine- N- (lipid-PEG LP). This finding could be used to formulate similar liposomes to a specific zeta potential, potentially of importance for systems sensitive to highly charged species. A strong linear relationship was noted between zeta potential values and the mole percentage of charged lipids within a liposome (e.g., cationic DOTAP or anionic DOPS). Liposomes were prepared with cholesterol, 1,2-distearoyl- sn-glycero-3-phosphocholine (DSPC), and varying amounts of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or 1,2-dioleoyl- sn-glycero-3-phospho- l-serine (DOPS). This study examines zeta potential dependence on pH and ionic strength, resolving power, and highlights the sensitivity of zeta potential to charged liposomes. This case study attempts to explore the sensitivity of zeta potential measurement using specifically formulated cationic, anionic, and neutral liposomes. Without a thorough understanding of the measurement parameters and limitations of the technique, these values can become meaningless. While useful, zeta potential values provide only very general conclusions about surface charge character. Zeta potential is often used to approximate a nanoparticle’s surface charge, i.e., cationic, anionic, or neutral character, and has become a standard characterization technique to evaluate nanoparticle surfaces.